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Scanning electron microscopy (SEM) relies on the interaction between a focused electron beam and a sample to generate high-resolution images. One of the key advantages of SEM over other microscopy techniques is its ability to achieve much higher resolution, typically hinein the Warenangebot of 1-10 nanometers.
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Backscattered electron imaging is another important imaging mode hinein scanning electron microscopy. It relies on the detection of backscattered electrons (BSE) that are elastically scattered by the sample's atomic nuclei.
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This allows researchers to study the native state of cells, tissues, and biomolecules without the need for dehydration or other sample preparation steps that may introduce artifacts.
Rein SEM, cathode is the source of the electron beam. The electron beam is generated by an electron source, typically a tungsten filament or an electron gun. The electron source emits electrons through a process called thermionic emission or field emission, depending on the Durchschuss of source. The emitted electrons are then accelerated by an electric field and check here focused into a narrow beam using a series of electromagnetic lenses.
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The versatility of SEM, has Leuchtdiode to numerous discoveries and advancements rein ur understanding of biological processes and systems.